Table 1.

Cell-cycle effects of XMT-1536.

TreatmentConcentration (nmol/L)Percentage of cells in G2–M
None034.3
XMT-15360.0139.9
XMT-15360.0545.4
XMT-15360.155
XMT-15360.573
XMT-1536171.1
None038.4
Bafilomycin A033.6
XMT-15360.145.6
XMT-1536 + Bafilomycin A0.131.8
XMT-15360.574.5
XMT-1536 + Bafilomycin A0.537.4
  • Note: OVCAR3 cells were treated with XMT-1536 ranging from 0 to 1 nmol/L and in some cases 30 nmol/L Bafilomycin A for 24 hours. The amount of nuclear DNA in each cell was quantified in 5,000 cells for each treatment by a MACSQuant flow cytometer (Miltenyi Biotec) using propidium iodide. The percentage of arrested cells was determined by dividing the number of cells in G2–M phase by the total number of cells and multiplying by 100.