Table 3.

Quantitation of activities of TriTACs with different affinities for CD3.

CD3KD humanKD cynoTDCCAUCHalf-life
TargetBinderCD3ε (nmol/L)CD3ε (nmol/L)EC50 (pmol/L)(h*nmol/L)(h)
MSLN2B244271589.4
2E414137
2A44043201,03084.9
PSMA2B24410
10B2181787
1G41331341,389
  • Note: Affinity of anti–CD3 scFvs for human and cynomolgus monkey (cyno) CD3ε was measured by biolayer interferometry. EC50 values for anti-MSLN and anti-PSMA TriTACs containing these anti–CD3 scFv tested in TDCC assays. AUC and half-life values calculated from measured serum exposures of two anti-MSLN TriTACs in cynomolgus monkeys after single 0.02 mg/kg i.v. bolus doses.