Table 1.

Summary of CD47 expression levels and binding properties of AO-176 and AO-104 to CD47 expressed on normal cells.

SpeciesCell typeMean CD47 RNEC50 AO-176 (ng/mL)EC50 AO-104 (ng/mL)Bmax AO-176Bmax AO-104
HumanRBCs32,086Negligible4–20645–9869,458–16,615
Platelets34,4391,0007–202,118–4,30112,613–69,415
Naïve CD3+ T cells83,4771,600–6,0002–1206,032–14,24522,492–54,972
Activated CD3+ T cellsN.D.3,000–26,000894,200–43,52121,996–91,383
Endothelial48,9443,000–7,1009–403,994–29,762168,816–264,242
Epithelial115,7843,3005–208,74346,956–250,699
Skeletal85,6612,2750.92,260226,204
Cynomolgus monkeyRBCs26,0875,000–10,00012–18675–1,7513,951–7,183
Platelets34,7281,000–5,80017–585,852–13,78118,866–40,265
CD3+ T cells35,8003,180–5,0933–73,652–8,92513,721–26,609