Table 2.

KLF4 mutations identified in ctDNA of patients with acquired cetuximab resistance and the prediction of SIFT and PolyPhen2.

Case numberGeneAmino acid changeSIFTaPolyPhen2b
1KLF4p.M475KDamaging0Probably damaging0.996
1KLF4p.T286PDamaging0.02Probably damaging0.988
5KLF4p.A472DDamaging0Probably damaging0.996
8KLF4p.A472DDamaging0Probably damaging0.996
  • aA smaller score of SIFT indicated damaging of protein function.

  • bA larger score of PolyPhen2 revealed a possible or probably damaging effect.