Table 3.

Radiation sensitivity and radiosensitizing effect of BRM knockdown for BRG1-deficient and -proficient cell lines from the clonogenic assay

Cell lineBRG1 mutationSurviving fraction of control cells at 2 GySurviving fraction of control cells at 4 GySurviving fraction of control cells at 6 GyAverage clonogenic survival-modifying factors for BRM knockdown at 2–6 Gy
H1299Yes0.69 (0.62–0.77)0.35 (0.31–0.39)0.143 (0.128–0.159)0.56 (0.50–0.63)****
A549Yes0.67 (0.60–0.74)0.34 (0.31–0.38)0.099 (0.089–1.110)0.68 (0.61–0.75)****
H661Yes0.61 (0.51–0.72)0.26 (0.22–0.31)0.086 (0.073–0.102)0.62 (0.51–0.75)***
HCC15Yes0.61 (0.57–0.64)0.23 (0.22–0.24)0.067 (0.063–0.071)0.66 (0.62–0.70)****
HCC827No0.58 (0.54–0.64)0.27 (0.25–0.29)0.046 (0.042–0.050)0.97 (0.91–1.04) n.s.
H520No0.40 (0.31–0.51)0.12 (0.10–0.15)0.015 (0.012–0.019)0.81 (0.63–1.03) n.s.
H596No0.40 (0.35–0.44)0.12 (0.11–0.13)0.013 (0.011–0.014)1.10 (0.92–1.32) n.s.
H460No0.39 (0.33–0.46)0.11 (0.09–0.12)0.011 (0.010–0.013)1.29 (1.09–1.53) n.s.
HDFANo0.15 (0.13–0.17)n.d.n.d.1.00 (0.85–1.18) n.s.
HDFNNo0.17 (0.16–0.18)n.d.n.d.0.99 (0.89–1.11) n.s.
  • NOTE: Least square estimates from the analysis of the log colony data with a general linear are given together with 95% confidence intervals.

  • ****(***), Effect of BRM knockdown on surviving fractions at radiation dose from 2 to 6 Gy is significant at α = 0.0001 (α = 0.001) in comparison with nontargeting control siRNA-transfected cells for the indicated cell line. Interaction effects between radiation dose and BRM knockdown were not significant for all cell lines. n.s., Survival-modifying factor according to BRM knockdown was not significantly different from 1.