Table 1.

Effect of bufalin on TP53, AR and selected AR-target genes, and top 30 upregulated genes (24-hour exposure) in LNCaP cells

Symbol10 nmol/L bufalin20 nmol/L bufalinMajor functionsPMID
GAPDH0.940.86Housekeeping gene, glycolysis
ACTB1.82.0β-actin, Housekeeping gene
ACTG11.21.6γ-actin1
ACTA21.61.4Smooth muscle actin
AR1.10.75AR
KLK3 (PSA)0.990.73AR target gene
KLK20.680.58KLK family member
KLK41.40.49KLK family member
NKX3-10.570.21AR target gene
SRA-10.910.79Steroid receptor RNA activator 1
TP531.21.0P53 tumor suppressor gene
CYR61/CCNI10.625.2Senescence, DDR, P53, ROS, P16 activator22566095
CTGF/CCN23.713.1Senescence, TGFβ target, cancer catabolism22684333
PPP1R15A/GADD343.810.0Senescence, DDR, P53 activator14635196
BCL66.07.4Senescence, P53 target, Apoptosis suppressor18524763
CDKN1A (P21Cip1)7.27.2Senescence, P53 target, DDR, cycle arrest8242752
HMOX12.714.3Cellular stress, ROS, P53 target17933770
DUSP17.511.2Cellular stress, ROS, P53 target18403641
DUSP53.15.3Cellular stress, ROS, P53 target12944906
TXNIP5.75.5Cellular stress, ROS, P53 activator23823478
IFRD1/PC4/Tis74.15.7Cellular stress, P53 activator17785449
SDC43.97.5Cellular stress, hypoxia15141079
TRIB13.85.9Cellular stress, ER cheperone, p53 inactivator25832642
ATF35.812.7Cellular stress sensor, P53 target21927023
TSC22D37.68.0Cellular stress sensor, osmotic17147695
SIPA1L23.96.6Cellular stress response22178795
IER53.27.3Cellular stress, chaperone, checkpoint23273978
RNF103/Kf-13.25.4Cellular ER stress, ubiquitin ligase18675248
NUPR1/Com1/p83.35.3Cellular stress, P53 interactor, P21 activator18690848
GADD45B7.213.6Growth arrest, p53 target23948959
GADD45A6.412.1DDR, p53 target10779360
BTG24.35.4DDR, P53 target, cycle arrest8944033
GDF15/NAG-19.07.6Tumor suppressor, P53 target23220538
EFNA13.97.4Tumor suppressor, antiproliferation17949899
ERRFI13.97.2Tumor suppressor, antiproliferation17351343
TNFRSF12A5.19.1AR-repressed metastasis gene24970477
ELF36.28.4AR repressor23435425
GABARAPL13.55.5Autophagy, maturation20404487
ISG20L12.15.6Autophagy, P53 target20429933
SLC38A25.26.6Solute transport21622135
KLHL283.36.2Ubiquitination regulator21145461
  • NOTE: Expressed as fold over control set as unity.