Table 2.

Summary of cell line characterization and in vitro activity

Flow cytometryCytotoxic activity
Cell linesAntigen density (±SEM)% PSMA-positive cells (±SEM)SG3199 (IC50 nmol/L ± SEM)MEDI3726 (IC50 nmol/L ± SEM)Isotype control ADC (IC50 nmol/L ± SEM)
LNCaP250,494 (1,229)92 (5)0.04 (0.01)0.02 (0.003)3.83 (1.01)
LNCaP C4-2250,446 (2,251)91 (8)0.16 (0.02)0.06 (0.01)7.47 (0.45)
CWR22Rv143,776 (4,237)40 (7)0.07 (0.01)0.29 (0.21)3.56 (0.36)
MDA PCa2b117,635 (5,200)95 (0)1.05 (0.36)0.002 (0.001)0.51 (0.09)
PC-3NegativeNA0.24 (0.10)29.3 (0.6)25.2 (1.55)
DU145NegativeNA0.26 (0.06)44.9 (7.7)37.4 (8.33)
  • NOTE: The cytotoxicity of SG3199, MEDI3726, and the isotype control ADC was determined by CellTiter-Glo assay. n = 3; NA = not applicable; Negative = below the lower limit of quantitation (7,348).