Table 1.

Cytotoxic activity of the domain II mutant immunotoxins

ConstructIC50 (pmol/L)Fold reduction in activity (compared with PE38)
LMB-2 (αTac-scFv-PE38)0.1
αTac-dsFv-PE380.1
αTac-dsFv-PE243.534
αTac-dsFv-M12-PE2430.2297
αTac-dsFv-M14-PE245.453
αTac-dsFv-M15-PE241.515
αTac-dsFv-M16-PE244.242
αTac-dsFv-M17-PE243.232
αTac-dsFv-M18-PE240.77
  • NOTE: Immunotoxins were tested for cytotoxicity in a cell growth inhibition assay on the HUT-102 strain. Averages of 3 to 6 assays are shown.