Table 2.

ABBV-221 cytotoxicity in human tumor cell lines

Tumor typeEGFR genotypeReceptor numberCytotoxicity (nmol/L)a
U87MGde2-7Glioblastoma multiformeEGFRvIII1 × 106 0.2
A431Vulvar epidermoid carcinomaWild-type2 × 106 1
HCC827.ER.LMCLung adenocarcinomaWild-type, E746_A7505.85 × 105 11
EBC1Lung squamous cell carcinomaWild-type1.84 × 105 69
NCI-H1703Lung squamous cell carcinomaWild-type2.8 × 105 4
NCI-H441Lung adenocarcinomaWild-type1.4 × 105 80
NCI-H292Lung adenocarcinomaWild-typeNDb 60
FaDuSquamous cell carcinoma of the hypopharynxWild-type4.77 × 105 8
LoVoColorectal adenocarcinomaWild-type1.28 × 105 8
HCT15Colorectal adenocarcinomaWild-type0.95 × 105 >1,000
SW620Colorectal adenocarcinomaEGFR-null->1,000
  • aCell viability was determined following incubation with ABBV-221 for 72 hours. The values represent IC50s. Unconjugated AM1 does not inhibit growth of any of these cell lines.

  • bND, not determined—an accurate number could not be obtained due to clumping of cells.