Table 1.

In vitro cytotoxicity of SGN-CD123A on AML cell lines

Mutationsa
Cell LineFLT3p53OtherMDR statusCD123 copy numberSGN-CD123A IC50, ng/mL
HNT-34NDBCR-ABL124,4000.2
MOLM-13+MLL-AF920,0000.08
THP-1MutantMLL-AF98,00024
EOL-1MLL-PTD9,000<0.0001
NOMO-1MutantMLL-AF97,00038
SKM-1Mutant6,6002.5
MV4-11+MLL-AF426,1000.02
KG1 (ATCC8031)MutantNRAS+9,4000.8
KG1 (ATCC246)MutantNRAS+5,0000.6
GDM-1Mutantt(6;7)+5,5002
Kasumi-1MutantAML1/ETO+2,0001
TF1-αMutantComplex+2,300>1,000
HEL 92.1.7MutantJAK2V617F+0>1,000
  • NOTE: In vitro cytotoxicity assays were 96-hour assays except for Kasumi and KG1 (ATCC246), which were 7-day assays. CD123 copy number was determined by quantitative flow cytometry using a commercial kit.

  • Abbreviation: ND, not determined.

  • aWellcome Trust Sanger Institute (COSMIC) and DSMZ Guide to Leukemia and Lymphoma Cell Lines.