Table 1.

EC50 values of cell death induction

MoleculeColo205HCT116
w/o BZB+ BZBw/o BZB+ BZB
scTRAIL474.7 ± 139.322.4 ± 2.31122.7 ± 353.18.2 ± 1.5
Db-scTRAIL40.9 ± 3.03.7 ± 0.434.9 ± 2.41.7 ± 0.8
EHD2-scTRAIL34.5 ± 6.82.9 ± 0.475.0 ± 13.51.3 ± 0.4
scFv-EHD2-scTRAIL12.6 ± 2.61.8 ± 0.411.3 ± 1.70.7 ± 0.3
Fc-scTRAIL90.7 ± 3.57.0 ± 0.2165.7 ± 25.63.6 ± 1.5
scFv-Fc-scTRAIL12.8 ± 1.62.3 ± 0.512.5 ± 4.70.5 ± 0.1
  • NOTE: Effects on Colo205 and HCT116 cells were analyzed in the absence and presence of bortezomib (250 ng/mL). EC50 values (pmol/L) correspond to concentration of scTRAIL.

  • Abbreviation: BZB, bortezomib.