Table 2.

EC₅₀ values^a (nmol/L drug) for αCD30 glucuronide-based DAR 8 ADCs

ADC cytotoxicity		CD30⁺ lymphoma cell lines
8-drugs/antibody	Linker	L540cy, HL	L428^b, HL	Karpas299, ALCL	DEL, ALCL	DEL-BVR^b, ALCL	Ramos (CD30⁻)
αCD30–6	glucQ-AE	0.032 nmol/L	0.051	0.010	0.014	0.17	>100
αCD30–7	gluc-MMAE	0.086	0.16	0.015	0.035	0.72	>100
αCD30–8	glucQ-Tub	0.053	0.014	0.016	0.042	0.20	>100
αCD30–9	gluc-DMTub	0.27	1.4	0.18	0.11	6.0	>100

↵^aCells were treated for 96 hours, then assessed for cell viability as described in the Materials and Methods.
↵^bMDR-positive by rhodamine efflux.