Table 2.

EC50 valuesa (nmol/L drug) for αCD30 glucuronide-based DAR 8 ADCs

ADC cytotoxicityCD30+ lymphoma cell lines
8-drugs/antibodyLinkerL540cy, HLL428b, HLKarpas299, ALCLDEL, ALCLDEL-BVRb, ALCLRamos (CD30)
αCD30–6glucQ-AE0.032 nmol/L0.0510.0100.0140.17>100
αCD30–7gluc-MMAE0.0860.160.0150.0350.72>100
αCD30–8glucQ-Tub0.0530.0140.0160.0420.20>100
αCD30–9gluc-DMTub0.271.40.180.116.0>100
  • aCells were treated for 96 hours, then assessed for cell viability as described in the Materials and Methods.

  • bMDR-positive by rhodamine efflux.