Table 3.

Summary of results from different assays of active compounds

Docking results, ranking
CompoundKDa (μmol/L)Level of NK1 bindingb (%)Inhibition of tumorigenesiscLevel of cell migrationd (%)Pocket 1Pocket 2Pocket 3
663100Non.d.29624
1087960Non.d.1151
1510,54017Yes373312
2519260NoNo201028
2640660Yese60262729
2776100YeseNo71126
31248Yesn.d.141822
3380100NoNo23169
  • Abbreviation: n.d., not determined.

  • aAgainst Met567 in SPR.

  • bTo Met928 in competition assays at 1 mmol/L compound.

  • cActivity assessed in tumorsphere assay.

  • dActivity assessed in migration assay.

  • eWith additional antiproliferative properties.