Table 1.

Impact of ABC29460 on individual dihydroceramide species

ABC294640
Dihydroceramide speciesUntreated10 μmol/L20 μmol/L30 μmol/L
dhC140.244 ± 0.0260.603 ± 0.043a (2.5)0.801 ± 0.050a (3.3)1.295 ± 0.440 (5.3)
dhC161.107 ± 0.0513.036 ± 0.183b (2.7)3.424 ± 0.361a (3.1)5.952 ± 1.309a (5.4)
dhC180.017 ± 0.0010.054 ± 0.007a (3.1)0.072 ± 0.022 (4.2)0.280 ± 0.096 (16.3)
dhC18:10.006 ± 0.0020.016 ± 0.003 (2.8)0.018 ± 0.002a (3.0)0.056 ± 0.035 (9.8)
dhC200.066 ± 0.0050.094 ± 0.005a (1.4)0.147 ± 0.008a (2.2)0.462 ± 0.034b (7.1)
dhC20:10.005 ± 0.0040.006 ± 0.001 (1.2)0.014 ± 0.001 (2.6)0.054 ± 0.001b (10.3)
dhC220.267 ± 0.0241.044 ± 0.003c (3.9)1.253 ± 0.013c (4.7)1.746 ± 0.076b (6.5)
dhC22:10.040 ± 0.0000.101 ± 0.020a (2.5)0.145 ± 0.026a (3.7)0.321 ± 0.023b (8.1)
dhC241.243 ± 0.2125.674 ± 0.130b (4.6)6.830 ± 0.382b (5.5)4.471 ± 0.789a (3.6)
dhC24:10.361 ± 0.0701.336 ± 0.077a (3.7)1.280 ± 0.054b (3.5)1.024 ± 0.145a (2.8)
dhC260.031 ± 0.0070.106 ± 0.004a (3.4)0.153 ± 0.035a (4.9)0.090 ± 0.015a (2.9)
dhC26:10.049 ± 0.0220.147 ± 0.004a (3.0)0.139 ± 0.002a (4.2)0.970 ± 0.025 (4.6)

NOTE: Cells were treated with ABC294640 at the indicated concentrations and dihydroceramides measured at 72 hours. Units are the mean ± SD expressed in nmol dh-Cer/pmol lipid phosphate. Data shown are from a representative experiment performed in duplicate. Similar results were obtained in an independent experiment. Numbers in parenthesis indicate fold change compared to untreated control.