Table 1.

Binding affinities of 2B.1 antibodies for FGFR2-IIIb

Variant IDCDR-H2 sequenceTimes found (n)FGFR2-IIIb KD, nmol/LVariant IDCDR-H2 sequenceTimes found (n)FGFR2-IIIb KD, nmol/LVariant IDCDR-H2 sequenceTimes found (n)FGFR2-IIIb KD, nmol/L
R3MabIYPTN0ND2B.1.14LWYFD11.62B.1.72VHPFE13.5
2B.1.1YWAWD30.292B.1.71VWMFD11.62B.1.44WWSWG13.6
2B.1.88IWMFT40.642B.1.28FWAWS21.82B.1.52FSLGD13.9
2B.1.38FWAYD11.12B.1.95LIFFT21.82B.1.30VSFFS14.1
2B.1.20LDVFW11.22B.1.50LNFYS22.02B.1.82INFFS14.9
2B.1.32WVGFT91.22B.1.81VNNFY12.12B.1.93IDNYW135.1
2B.1.49LSFFS11.32B.1.25WHPWM12.32B.1.55VDVFW35.9
2B.1.86LSFWT11.32B.1.3THLGD12.62B.1.35WHPFR59.4
2B.1.9YHPYL81.42B.1.65YNAYT12.72B.1.33YHPFH215
2B.1.73MIFYN11.42B.1.94LVFFS33.12B.1.80YWAFS217
2B.1.74YHPFR11.42B.1.78LSFYS43.22B.1.92WVAFS2NA

NOTE: Residues that match those in R3Mab are underlined.

Abbreviations: NA, not available due to protein aggregation; ND, not detectable.