Table 1.

Resistance profiles of 361-TM and JIMT1-TM cell lines to ADCs

361: Parental versus TM-resistantJIMT1: Parental versus TM-resistant
ADC TreatmentLinker typeParental (IC50, nmol/L)TM (IC50, nmol/L)Relative resistanceParental (IC50, nmol/L)TM (IC50, nmol/L)Relative resistance
T_MCC_DM1 (TM-ADC)NC1.6410256×5282016×
T_mc'_Aur-8261NC0.41>1,000>2,439×10>1,000>100×
T_Mal'Peg6C2_ MMADNC3.0800267×52>1,000>19×
T_vc_MMADC0.500.290.6×1356043×
T_vc_Aur-8254C0.200.201.0×4.8880183×

NOTE: Parental and TM-resistant cell lines were treated with the indicated ADCs and cytotoxicity assessed as indicated in Materials and Methods. Data are mean IC50 from multiple experiments (n = 2–26), rounded to two significant figures. Relative resistance is the ratio of the mean IC50 for the TM-resistant cell line versus the corresponding parental cell line.

Abbreviations: C, cleavable linker; NC, noncleavable linker.