Table 1.

Domain specificity and binding kinetics for selected antibodies binding human HER2

AntibodyHER2 domainkon (per mol/L per s)koff (per s)KD (nmol/L)
4385a121.2 × 1043.0 ± 0.05
4387a118.3 × 1043.3 ± 0.07
4380114.3 × 1041.9 × 10−41.3 ± 0.04
438319.2 × 1041.1 × 10−41.1 ± 0.02
438227.2 × 1041.1 × 10−41.5 ± 0.02
Pertuzumabb223.4 × 1040.8 × 10−40.3 ± 0.01
4384323.7 × 1041.2 × 10−40.5 ± 0.02
4518420.7 × 1049.4 × 10−44.6 ± 0.08
4517416.9 × 1043.7 × 10−42.2 ± 0.03
Trastuzumabc428.3 × 1041.2 × 10−40.4 ± 0.01
  • aApparent affinity.

  • b0.8 nmol/L (Australian Public Assessment Report for Pertuzumab. December 2013. http://www.tga.gov.au).

  • c0.5 nmol/L (Bostrom et al. Plos ONE 2011;6:1–12).