Table 1.

TDP1 and TOP1 staining in tumors selected from different colorectal anatomic sites

TDP1 staining
NoneLowMediumHigh
Ascending1483
Cecum0210
Descending0131
Rectum0322
Sigmoid1497
Transverse0241
TOP1 staining
NoneLowMediumHigh
Ascending0484
Cecum0210
Descending0121
Rectum0033
Sigmoid04710
Transverse0232

NOTE: TMA slides of paraffin-embedded colorectal cancer samples with documented tumor demographic and correlated overall survival data were deparaffinized and stained with antibodies for TDP1 (NB100-81642; Novus Biologicals) and TOP1 (NCL-TOPO1; Leica Microsystems), both at 1:200 dilution using a Bond Max Autostainer. Scores were classified as none, low, moderate, or high for TDP1 and TOP1 staining, with samples recorded if >5% cells stained accordingly.