Table 2.

Effects of CGP-2 and temozolomide treatment on NS-IC assay

NS-IC frequency controlNS-IC frequency after CGP-2 treatmentNS-IC frequency after temozolomide treatment
GIC-21/17 (±4)1/735 (±28; aP < 0.05)1/735 (±46; aP < 0.05)
GIC-31/25 (±3)1/321 (±55; bP < 0.001)1/164 (±6)
GIC-61/16 (±1)1/1710 (±725; bP < 0.001)1/661 (±16; aP < 0.05)
GIC-121/42 (±5)1/86 (±2; aP < 0.05)1/63 (±2)

NOTE: Treatment with CGP-2 and temozolomide resulted in a significant decrease of NS-IC frequency (a, P < 0.05; b, P < 0.001; calculated with a two-tailed unpaired Student t test). Final cell dilutions yielding 37% of negative wells correspond to the dilution at which there is one NS-IC per well (mean ± SD).