Table 1.

Mesothelin expression and cytotoxicity of SS1P and RG7787 in PDAC cell lines

IC50 ± SEM (ng/mL)IC50 ± SEM (pmol/L)
Cell linesMesothelin binding sites/cell (×103)SS1PRG7787SS1PRG7787
GUMC108353.98 ± 0.491.38 ± 0.1562.8 ± 6.118.9 ± 2.0
KLM-16023.90 ± 2.814.44 ± 0.41382.4 ± 45.060.9 ± 5.6
AsPC-13014.76 ± 2.2422.5 ± 1.34236.1 ± 35.9308.7 ± 18.4
BxPC-3324.4 ± 2.4733.28 ± 6.47390.2 ± 39.47638.5 ± 114.88
Panc 3.01490848.2 ± 151.8108.5 ± 2.413,573.4 ± 2,429.81,489 ± 30.71
PK-15638.5 ± 11.9>100010,217.7 ± 1,838.4>13,721.6

NOTE: Average data from at least three independent experiments are displayed. Mesothelin expression is evaluated by flow cytometry, and IC50s are derived from 72-hour ATP cell viability assays. Cell lines are ranked from most to least sensitive to RG7787.