Table 1.

Kinetics parameters

BufferKM (nmol/L)kcat (per second)kcat/KM (L/mol per second)
HTS buffer1 × PBS, PH 7.4
80 mmol/L KCl3,936112.8 × 106
0.01% Tween-20
WCE buffer50 mmol/L Tris-HCL, pH 7.5
80 mmol/L KCl
2 mmol/L EDTA80787.5 × 106
1 nmol/L DTT
40 μg/mL BSA
0.01% Tween-20