Table 1.

CD33 surface expression and EC50 values for AMG 330–mediated redirected lysis

Cell lineCD33 surface molecules per cellEC50 (pg/mL)
U-93714,40023.3
M-07e15,400117.8
KASUMI-116,20059.1
NB-416,60076.5
NOMO-120,300132.4
EOL-121,200148.8
SKM-129,500102.9
KG-135,90018.5
THP-137,40033.8
HL-6045,50027.4
MOLM-1356,70031.4

NOTE: Number of CD33 surface molecules on human AML cell lines was determined using the DAKO QUIFIKIT and the anti-CD33 monoclonal antibody clone WM53. EC50 values for redirected lysis of human AML cell lines after 48 hours at an E:T-cell ratio of 5:1 were calculated from dose–response curves using GraphPad Prism software.