Binding affinities of 1 (UMI-59) and 2 (UMI-77) to five antiapoptotic Bcl-2 family members in competitive FP assay using fluorescent-labeled Bid BH3 peptide
| Ki ± SD, μmol/L | ||
|---|---|---|
| Protein | 1 (UMI-59) | 2 (UMI-77) |
| Mcl-1 | 1.55 ± 0.18 | 0.49 ± 0.06 |
| A1/Bfl-1 | 6.14 ± 1.0 | 5.33 ± 1.0 |
| Bcl-2 | 54.65 ± 9.56 | 23.83 ± 1.81 |
| Bcl-xL | 99.0 ± 22.63 | 32.99 ± 4.33 |
| Bcl-w | 37.53 ± 7.96 | 8.19 ± 1.91 |