Table 1.

Identification of proteins differentially expressed in drug-resistant cells

Fold changea
A549 cell seriesHey cell series
NameCodeEpoB40AT12EpoB8Ixab80
Chloride intracellular channel protein 1CLIC13.2 ± 0.4b−(1.8 ± 0.4)−(1.7 ± 0.3)
Stathmin (right spot, pI 6.0)STMN1
Stathmin (left spot, pI 5.5)STMN1−(2.4 ± 0.5)c−(1.5 ± 0.4)−(1.8 ± 0.7)
β-/γ-ActinACTB/G2.3 ± 1.2
β1-Tubulin (Gln to Glu)TBB3.5 ± 0.6
KeratinKRT2.2 ± 0.8
VimentinVIME2.0 ± 0.6
14-3-3 σ1433S2.1 ± 0.6
EzrinEZR12.1 ± 0.5
Galectin-1LEG12.3 ± 0.31.9 ± 0.41.8 ± 0.4
Metastasis inhibition factor NM23NM23H1−(2.1 ± 0.5)−(2.0 ± 0.2)
Heat shock protein HSP90HSP902.3 ± 0.6
Glucose-regulated protein 78GRP782.0 ± 0.6
Heat shock protein HSP27HSP272.7 ± 1.21.9 ± 0.7
Carbonic anhydrase 2CA2−(3.1 ± 1.5)

NOTE: Data were obtained after 2D DIGE coupled with MALDI TOF/TOF mass spectrometry analysis.

  • aFold change: the expression level of proteins in the drug-resistant cells compared with the drug-sensitive cells. Those numbers not in bold have a mean fold change of less than 2.

  • bMean ± SD, n = 3.

  • c“Minus” values represent fold decrease in protein expression.