Table 1.

Sequences and TRAILR2 binding affinities of Tn3 variants

Sequence
CloneBC loopDE loopFG loopKd (nmol/L)Kd 1C12/Kd mutant
Wild-typeFKPLAEIDGTEDENQRRGDMSSNPA
1C12AKPEKWDGSIYGNSRHTAFTPYGAKSNPA4130 ± 281
G3AKPEKWDPPLWGNSRHTAFTPYGAKSNPA422 ± 4510
1E11AKPWVDPPPLWGQQKHTAFDPYGAKSNPA103 ± 940
C4AKPWVDPPPLWGQQKHTAFDPYNKRNVPA50 ± 283
G6AKPWVDPPPLWGQQKHTAFDPYGMRSKPA43 ± 296
D1SPGERIWMFTGTEDENQPNYERISNPAno binding

NOTE: Loop sequences are shown for each Tn3 clone. 1C12 and its derivatives (G3, 1E11, C4, and G6) contained a Thr -> Ala point mutation within the framework at position 29 (see Materials and Methods for Tn3 sequence numbering). Underlined sequence denotes changes relative to 1C12 for affinity optimized variants. Clone D1 is a control Tn3 that binds to the antibody palivizumab.