Table 1.

Comparison of affinities of scFv, Fab, and IgG for binding to EGFR-overexpressing A431 cells

AntibodyscFv KD, nmol/LFab KD, nmol/LIgG KD, nmol/LKD (Fab)/KD (IgG)
C10263.67124.231.17106
P3/588.2458.240.5116
P2/114.8125.40.0122,117
P2/415.3925.20.00643,938
P2/217.0118.10.00772,351
35247.4715.40.0121,903
21249.901.310.007187
22240.941.20.007171
C225NA0.0130.0062

NOTE: KD values for binding to cells were determined by fitting the data from Fig. 1 to the Lineweaver–Burk equation.

Abbreviation: NA, not applicable.