Comparison of affinities of scFv, Fab, and IgG for binding to EGFR-overexpressing A431 cells
| Antibody | scFv KD, nmol/L | Fab KD, nmol/L | IgG KD, nmol/L | KD (Fab)/KD (IgG) |
|---|---|---|---|---|
| C10 | 263.67 | 124.23 | 1.17 | 106 |
| P3/5 | 88.24 | 58.24 | 0.5 | 116 |
| P2/1 | 14.81 | 25.4 | 0.012 | 2,117 |
| P2/4 | 15.39 | 25.2 | 0.0064 | 3,938 |
| P2/2 | 17.01 | 18.1 | 0.0077 | 2,351 |
| 3524 | 7.47 | 15.4 | 0.012 | 1,903 |
| 2124 | 9.90 | 1.31 | 0.007 | 187 |
| 2224 | 0.94 | 1.2 | 0.007 | 171 |
| C225 | NA | 0.013 | 0.006 | 2 |
NOTE: KD values for binding to cells were determined by fitting the data from Fig. 1 to the Lineweaver–Burk equation.
Abbreviation: NA, not applicable.