Comparison of measured apparent IgG affinities for A431 cells versus affinities calculated by using a mathematical model
| IgG | Experimental KD, nmol/L | Model KD, nmol/L | Model KD (equilibrium), nmol/L | KD (Fab)/KD (IgG) |
|---|---|---|---|---|
| C10 | 1.17 | 1.5 | 1.5 | 82.8 |
| P3/5 | 0.5 | 0.054 | 0.054 | 1,078 |
| P2/1 | 0.012 | 0.0071 | 0.0051 | 4,980 |
| P2/4 | 0.0064 | 0.0071 | 0.0051 | 4,941 |
| P2/2 | 0.0077 | 0.0059 | 0.0034 | 5,323 |
| 3524 | 0.012 | 0.0057 | 0.0026 | 5,923 |
| 2124 | 0.007 | 0.0053 | 0.00033 | 3,969 |
| 2224 | 0.007 | 0.0053 | 0.00031 | 3,870 |
NOTE: Fab affinities from Table 1. KD (Fab)/KD (IgG) was corrected for nonequilibrium conditions.