Table 2.

Comparison of measured apparent IgG affinities for A431 cells versus affinities calculated by using a mathematical model

IgGExperimental KD, nmol/LModel KD, nmol/LModel KD (equilibrium), nmol/LKD (Fab)/KD (IgG)
C101.171.51.582.8
P3/50.50.0540.0541,078
P2/10.0120.00710.00514,980
P2/40.00640.00710.00514,941
P2/20.00770.00590.00345,323
35240.0120.00570.00265,923
21240.0070.00530.000333,969
22240.0070.00530.000313,870

NOTE: Fab affinities from Table 1. KD (Fab)/KD (IgG) was corrected for nonequilibrium conditions.