qRT-PCR–based screens reveal 18 compounds inhibiting the expression of MELK, ASPM, TOP2A, and FOXM1b
| Library | Compound ID | MELK | ASPM | TOP2A | FOXM1B |
|---|---|---|---|---|---|
| ChemBridge | #5560509 | −2.1 | −3.5 | −3.2 | −1.5 |
| ChemBridge | #5256360 | −2.6 | −1.4 | −4.0 | −2.0 |
| Prestwick | Camptothecine (S,+) | −2.3 | −1.5 | −0.2 | −0.5 |
| ChemBridge | #5402594 | −0.2 | −1.8 | −2.4 | −0.5 |
| ChemBridge | #5551547 | −0.8 | −1.8 | −2.0 | 0.0 |
| ChemBridge | #5349968 | −0.7 | −0.2 | −3.2 | −4.3 |
| ChemBridge | 5356272 | −0.5 | −1.0 | −2.1 | −2.3 |
| ChemBridge | #5354001 | −0.7 | −1.1 | −1.7 | −2.6 |
| ChemBridge | #5194403 | −0.1 | −0.5 | 1.0 | −3.6 |
| ChemBridge | #5212518 | −0.1 | −0.3 | −1.2 | −2.2 |
| ChemBridge | #5316908 | −0.6 | −0.7 | −1.1 | −1.6 |
| BIOMOL | Ro 31-8220 | −2.7 | 0.8 | 0.3 | 1.2 |
| BIOMOL | C8 Ceramine | −1.9 | 0.2 | −0.7 | −0.4 |
| BIOMOL | 6-Formylindolo[3,2-B]Carbazole | −1.7 | 0.6 | 0.6 | −1.0 |
| Prestwick | Chelidonine monohydrate (+) | −0.2 | −1.9 | −1.2 | 0.1 |
| Prestwick | Methiazole | −1.1 | −1.5 | −0.5 | 0.1 |
| ChemBridge | #5217497 | 1.7 | 1.5 | 0.1 | −1.8 |
| ChemBridge | #5241816 | −0.3 | −0.7 | −1.4 | −1.7 |
NOTE: Degree of gene expression downregulation is expressed as Z scores; Z < −1.5 were considered hits (bold).