Table 5.

Percentages of cells in cell cycle of G1, S, and G2–M phases of each group.

KHOSU-2OS
KHOSKHOS/miR-NullKHOS/miR-199a-3pU-2OSU-2OS/miR-NullU-2OS/miR-199a-3p
G1 (%)40.838.849.040.440.755.2
S (%)43.444.333.438.636.628.6
G2–M (%)13.915.314.821.721.515.1

NOTE: KHOS and U-2OS cell lines were transfected with miR-199a-3p precursor or control vector (miR-Null), stable clones were selected in 2 μg/ml of puromycin, and flow cytometry analysis of cell cycle was carried out as described in Materials and Methods.