Table 1.

The effect of Raf inhibitor C-1 on signaling and viability in cell lines with WT and mutated MAPK pathway genes

CellsMutation statusCell assay IC50 (╬╝mol/L)
B-RafRASP-ERKCell viability
A375V600EWT0.0020.31
WM-266-4V600DWT0.0030.04
SK-MEL-28V600EWT0.0030.11
Colo-205V600EWT0.0140.15
Sk-Mel2WTQ61RNRAS0.260.29
M24metWTQ61RNRAS0.851.80
A549WTG12SKRAS4.962.84
HCT-116WTG13DKRAS0.450.72
MIA PaCa-2WTG12CKRAS5.702.18
A431WTWTND2.44
HeLa + EGFWTWT4.201.70
NHF + bFGFWTWT2.701.64

Abbreviations: NHF, normal human fibroblast; bFGF, basic fibroblast growth factor; ND, not done.