Table 1.

Primers and reaction conditions of regular and real-time RT-PCR

GenesGenbank accession no.PrimersTemperature (°C)Size (bp)
E1A
    SenseAY3398655′-TATGATTTAGACGTGACG-3′60267
    Anti-sense5′-CTCGTCGTCACTGGG-3′
p53
    SenseM146945′-CATCTACAAGCAGTCACAGCA-3′61186
    Anti-sense5′-GGCGGCTCATAGGGCACCACCACA-3′
HER-2/Neu
    SenseM120365′-CATGGGAGAACTCTGAGTG-3′62182
    Anti-sense5′-CTCCTCACCCACCCCTCCCAACAC-3′
β-Actin
    SenseEF1562765′-ACCACACTTTCTACAATGA-360178
    Anti-sense5′-CCAGAGGCATACAGGGACAACACA-3′