Table 2.

Effect of paclitaxel on the dynamic instability of microtubules in βIII-tubulin knockdown cells and control siRNA–transfected cells

ParameterControl siRNAβIII-tubulin siRNA
Nontreated1.6 nmol/L PXL6 nmol/L PXL20 nmol/L PXLNontreated1.6 nmol/L PXL6 nmol/L PXL20 nmol/L PXL
Growth rate (μm/min)10.4 ± 0.5NS21.7*38.910.6 ± 0.211.719.023.9
Growth length (μm)1.4 ± 0.1NSNS36.31.5 ± 0.1NSNSNS
Shortening rate (μm/min)15.2 ± 0.413.4*22.654.716.4 ± 0.513.9*15.7*24.9
Shortening length (μm)1.7 ± 0.1NS22.0*49.41.8 ± 0.1NSNS19.9*
Catastrophe/min3.9 ± 0.2NS23.0*43.43.7 ± 0.1NSNSNS
Rescue/min6.5 ± 0.3NSNSNS6.4 ± 0.4NSNSNS
Catastrophe/μm0.6 ± 0.03NSNS89.80.7 ± 0.0424.7*NSNS
Rescue/μm0.5 ± 0.03NS44.8125.20.5 ± 0.03NSNS37.9*
% Growing48.1 ± 1.5NSNS44.246.1 ± 1.7NSNSNS
% Shortening31.4 ± 1.2NSNSNS30.6 ± 1.3NSNSNS
% Paused20.5 ± 0.7NS34.0*126.423.2 ± 1.6NSNSNS
Dynamicity (μm/min)9.6 ± 0.4NS30.564.910.1 ± 0.320.916.7*24.4