Table 1.

Effect of vincristine on the dynamic instability of microtubules in βIII-tubulin knockdown cells and control siRNA–transfected cells

ParameterControl siRNAβIII-tubulin siRNA
Nontreated2 nmol/L VCR10 nmol/L VCR40 nmol/L VCRNontreated2 nmol/L VCR10 nmol/L VCR40 nmol/L VCR
Growth rate (μm/min)10.4 ± 0.5NSNS65.9*10.6 ± 0.219.6*27.9*35.4*
Growth length (μm)1.4 ± 0.1NCNS67.0*1.5 ± 0.119.132.538.7*
Shortening rate (μm/min)15.2 ± 0.4NS17.170.2*16.4 ± 0.520.735.5*43.2*
Shortening length (μm)1.7 ± 0.1NSNS68.8*1.8 ± 0.121.434.244.0*
Catastrophe/min3.9 ± 0.2NS17.479.0*3.7 ± 0.1NS31.537.3*
Rescue/min6.5 ± 0.3NSNSNS6.4 ± 0.4NSNSNS
Catastrophe/μm0.6 ± 0.03NS51.3133.3*0.7 ± 0.0432.058.772.3*
Rescue/μm0.5 ± 0.03NS32.3149.2*0.5 ± 0.0352.2102.0*130.3*
% Growing48.1 ± 1.5NS12.776.4*46.1 ± 1.7NS30.328.8
% Shortening31.4 ± 1.2NSNS67.8*30.6 ± 1.3NS21.925.1
% Paused20.5 ± 0.719.747.3*286.3*23.2 ± 1.632.790.0*91.3*
Dynamicity (μm/min)9.6 ± 0.4NS27.989.4*10.1 ± 0.331.2*50.3*59.1*