Table 1.

AMG 102 binding results for chimeric and mutant HGF molecules

HGF chimera or mutant designation(Domains or amino acid numbers)AMG 102 binding
Human HGFMouse HGF
Human full length*N, K1, K2, K3, K4, βNoneYes
Mouse full lengthNoneN, K1, K2, K3, K4, βNo
Ch 1 HGFK1, K2, K3, K4, βN-domainYes
Ch 2 HGFK2, K3, K4, βN, K1Yes
Ch 3 HGFK3, K4, βN, K1, K2Yes
Ch 7 HGFN, K1, K2, K3, K4, β (586 to 728)β 507 to 585No
Ch 8 HGFβ (507 to 585)N, K1, K2, K3, K4, β (586 to 728)Yes
G555E HGFN, K1, K2, K3, K4, β555No
C561R HGFN, K1, K2, K3, K4, β561No
D592N HGFN, K1, K2, K3, K4, β592Yes
N601S HGFN, K1, K2, K3, K4, β601Yes
R647Q HGFN, K1, K2, K3, K4, β647Yes
Human NK (insertion) HGFN, K1, K2, K3, K4, β2 amino acid insertion of mouse 540 to 541No
Mouse NK (deletion) HGFNoneN, K1, K2, K3, K4, β (540, 541 deleted)No

Abbreviations: N, amino terminus; K1, K2, K3, and K4, the four conserved kringle domains of HGF; NK, insertion or deletion of Asn-Lys; Ch, chimera.

  • *Human HGF sequence, GenBank NM_000601.

  • Mouse HGF sequence, GenBank NM_010427.

  • Mutationally altered domain or amino acid substitution.