Table 1.

Cannabinoid modulation of cell cycle

Treatment, μmol/LMean (G0-G1)Mean (S)Mean (G2-GM)
CBD 0.41.08* ± 0.030.90 ± 0.050.97 ± 0.28
Δ9-THC 1.71.12* ± 0.040.78 ± 0.111.28 ± 0.37
Δ9-THC 1.7/CBD 0.41.23*, ± 0.020.49*, ± 0.082.69*, ± 0.56

NOTE: Cell cycle was measured using PI staining and FACS analysis, and Modfit was used to determine the percentage of cells in G0-G1, S, and G2-GM phase. U251 cells were treated for 3 d with CBD (0.4 μmol/L), Δ9-THC (1.7 μmol/L), or a combination of CBD (0.4 μmol/L) and Δ9-THC (1.7 μmol/L). The percentage of cells in each compartment was standardized by dividing it by the average percentage for the vehicle. This procedure was carried out for data from each experiment on each day. Statistical analysis was done as described in the Material and Methods.

Abbreviation: CBD, cannabidiol.

  • *P < 0.05.

  • Significant interaction.