Table 1.

Effects of ML-133 on growth of cancer cell lines and KLF4 gene expression

Cell lineType of cancerGrowth inhibition IC50 (μmol/L)KLF4 induction (fold increase)
HT-29Colon0.716.5
HCT-116Colon0.205.9
H-460Lung2.903.4
DU-145Prostate0.359.2
PC-3Prostate0.302.3
MDA-MB-231Breast0.501.5
MDA-MB-435Breast0.359.1
MOLT-4Leukemia0.42>10*
CCRF-CEMLeukemia0.15>10*
SK-MEL-2Melanoma0.202.4

NOTE: Growth inhibition (IC50 values in μmol/L) and KLF4 induction after 16-h treatment (gene expression measured by real-time PCR relative to DMSO-treated control) in various cancer cell lines treated with ML-133.

  • *KLF4 induction cannot be accurately determined due to the low basal levels of KLF4 in these cell lines.