Table 2.

Biological characteristics and sensitivity to MLN8054 in human neuroblastoma cell lines

Cell lineOriginMYCNp53 statusCell typeIC50 of MLN8054 (μmol/L)
SK-N-SHNB tumorSCWTN + S0.46 ± 0.01 (2)
SH-SY5Y-LucNB tumorSCWTN0.24 ± 0.02 (4)
IMR-32NB tumorAWTN0.30 ± 0.08 (2)
LAN-1NB tumorANullN0.67 ± 0.04 (4)
JFNB tumorAN/DN0.26 ± 0.06 (3)
SH-EPNB tumorSCWTS0.92 (1)
NB19NB tumorAWTS0.50 (1)
SMS-KCNNB tumorAN/DS2.77 ± 0.18 (2)
SK-N-ASNB tumorSCmtS4.11 ± 1.03 (2)
MCF-10ABreast normal epithelialWT12.92 (1)
MCF-7Breast tumorWT0.44 (1)

NOTE: Numbers represent average ± SD IC50 derived from a Cell Counting Kit-8 proliferation assay. Values in parentheses represent the number of experiments completed for each cell line.

Abbreviations: NB, neuroblastoma; SC, single copy; A, amplified; WT, wild-type; mt, mutated; N/D, not described; N, neuroblastic; S, substrate adherent.