Table 2.

Caspase activities in cell lysates treated with TZD18 (30 μmol/L) for different durations

Cell linesCaspasesDays of treatment
1234
MCF-7Caspase 81.2 ± 0.21.8 ± 0.32.2 ± 0.33.1 ± 0.4
Caspase 91.1 ± 0.11.5 ± 0.23.2 ± 0.44.4 ± 0.5
Caspase 3N.D.N.D.N.D.N.D.
MDAMB-231Caspase 81.1 ± 0.12.0 ± 0.32.8 ± 0.33.9 ± 0.5
Caspase 91.2 ± 0.11.8 ± 0.23.7 ± 0.45.8 ± 0.4
Caspase 31.0 ± 0.11.3 ± 0.14.6 ± 0.36.9 ± 0.6

NOTE: Cells were cultured either in the presence or absence of TZD18 (30 μmol/L) for different durations, washed, and lysed in lysis buffer. Caspase-8, caspase-9, and caspase-3 activities in the cell lysates were measured as described in Materials and Methods. Results are expressed as fold increase of optical density values compared with control (without treatment). Data are expressed as mean ± SD of triplicate experiments.

Abbreviation: N.D., not detectable.