List of genes involved in UPR and induced by TZD18 treatment in either MCF-7 or MDA-MB-231 cells
| Gene name | Genbank | Description | Fold change | |
|---|---|---|---|---|
| MDA-MB-231 | MCF-7 | |||
| GDF15 | AF003934 | Growth differentiation factor 15 | 17.67 | 15.29 |
| DDIT3 | BC003637 | DNA-damage-inducible transcript 3 (CHOP/GADD153) | 3.37 | 12.39 |
| DDIT4 | NM_019058 | DNA-damage-inducible transcript 4 | 3.21 | 10.35 |
| HSPA1A | NM_005345 | Heat shock 70 kDa protein 1A | 7.37 | 2.37 |
| GADD45A | NM_001924 | Growth arrest and DNA-damage-inducible, α | 5.1 | 6.14 |
| CDKN1A | NM_000389 | Cyclin-dependent kinase inhibitor 1A (p21, Cip1) | 1.7 | 6.13 |
| HSPA1B | NM_005346 | Heat shock 70 kDa protein 1B | 4.23 | 1.28 |
| TNFRSF-10B (DR5) | AF016266 | Tumor necrosis factor receptor superfamily, member 10 b (death receptor 5, DR5) | 2.3 | 4.12 |
| ATF3 | NM_001674 | Activating transcription factor 3 | 2.56 | 3.58 |
| HERPUD1 | AF217990 | Homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1 | 2.89 | 4.99 |
| HSPA5 | AF216292 | Heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa) | 2.68 | 2.79 |
| ATF4 | NM_001675 | Activating transcription factor 4 (tax-responsive enhancer element B67) | 2.4 | 2.45 |
NOTE: MCF-7 and MDA-MB-231 cells were treated with TZD18 (30 μmol/L) for 24 h. After incubation, total RNA was isolated and applied to microarray analysis as described in Materials and Methods. Genes that changed ≥2-fold in their expression levels by TZD18 were selected using GeneSpring software. Several genes that closely related to endoplasmic reticulum stress signaling are listed.