Table 1.

List of genes involved in UPR and induced by TZD18 treatment in either MCF-7 or MDA-MB-231 cells

Gene nameGenbankDescriptionFold change
MDA-MB-231MCF-7
GDF15AF003934Growth differentiation factor 1517.6715.29
DDIT3BC003637DNA-damage-inducible transcript 3 (CHOP/GADD153)3.3712.39
DDIT4NM_019058DNA-damage-inducible transcript 43.2110.35
HSPA1ANM_005345Heat shock 70 kDa protein 1A7.372.37
GADD45ANM_001924Growth arrest and DNA-damage-inducible, α5.16.14
CDKN1ANM_000389Cyclin-dependent kinase inhibitor 1A (p21, Cip1)1.76.13
HSPA1BNM_005346Heat shock 70 kDa protein 1B4.231.28
TNFRSF-10B (DR5)AF016266Tumor necrosis factor receptor superfamily, member 10 b (death receptor 5, DR5)2.34.12
ATF3NM_001674Activating transcription factor 32.563.58
HERPUD1AF217990Homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 12.894.99
HSPA5AF216292Heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa)2.682.79
ATF4NM_001675Activating transcription factor 4 (tax-responsive enhancer element B67)2.42.45

NOTE: MCF-7 and MDA-MB-231 cells were treated with TZD18 (30 μmol/L) for 24 h. After incubation, total RNA was isolated and applied to microarray analysis as described in Materials and Methods. Genes that changed ≥2-fold in their expression levels by TZD18 were selected using GeneSpring software. Several genes that closely related to endoplasmic reticulum stress signaling are listed.