Table 2.

Summary of MGMT activity, PARP activity, and ABT-888 potentiation of TMZ in primary pediatric cells

Cell IDLeukemia subtypeMSIMGMT activity (fmol/mg protein)PARP activity (fmol/μg protein)*ABT-888 PF
p115AMLS2,580ND4.6
p151AMLND1,2006703.2
p120AMLSUD3701.2
BM1261AMLND151UD1
p145Pre-B ALLND1,6901,3201
p152Pre-B ALLS3253501
p157Pre-B ALLS1,2601,1801
p158Pre-B ALLND3,2501,8601
BM1169Pre-B ALLND310401
BM1339Pre-B ALLND1,110901.2
BM1350Pre-B ALLND550701.5
p153T-ALLND1,5804301
p154T-ALLND1,7603101

Abbreviations: S, MSI stable; ND, not determined; UD, undetectable.

  • *MGMT activity <100 fmol/mg protein; PARP <40 fmol/μg protein.

  • IC50 TMZ/IC50 TMZ + ABT-888. Value of 1 indicates no potentiation.