Table 1

Cytotoxicity of various arsenic formulations to tumor cells

Cell linesIC50 (As/μmol/L)
As2O3Lip(Ni, As)f-Lip(Ni, As)f-Lip(Ni, As) + 2 mmol/L FA
KB (FR+)
    3 h>200>2007.1 ± 3.8>200
    12 h24.3 ± 7.432.4 ± 9.93.5 ± 0.250.4 ± 26.6
    24 h6.0 ± 0.812.1 ± 0.62.1 ± 0.113.5 ± 1.1
    96 h3.4 ± 0.64.4 ± 0.61.2 ± 0.45.6 ± 0.5
HeLa (FR+)
    3 h>200>20023.4 ± 2.4>200
    12 h18.3 ± 5.043.2 ± 14.09.3 ± 5.752.0 ± 5.9
    24 h6.0 ± 0.516.6 ± 9.15.5 ± 2.212.3 ± 0.5
    96 h4.3 ± 0.35.7 ± 0.41.8 ± 0.63.5 ± 0.8
MCF-7 (FR)*
    3 h>100>100>100
    12 h5.8 ± 0.420.9 ± 1.415.5 ± 7.7
    24 h3.2 ± 0.45.9 ± 0.55.4 ± 0.3

NOTE: Cells were incubated with drugs for a continuous 96 hours or exposed to drugs for 3, 12, and 24 hours at 37°C, then washed and further incubated up to 96 h in a drug-free medium followed by Guava ViaCount assay for IC50 measurement. IC50 values are based on As concentration (μmol/L). The mean values and SDs are based on two to four independent experiments.

  • *IC50 measurement was not done for a continuous 96-h incubation.