Table 1.
(A) Summary of mice i.p. injected with ID8 cells
PBSLPA
Tumor injection routei.p.i.p.
LPA injection routePumps + daily injectionPumps + daily injection
No. micen = 19n = 21
From 4 independent experimentsFrom 4 independent experiments
Tumors >0.05 mm3None3-5/mouse, 6-50 mm3
Average ascites forming time and volume80-100 d60-70 d
6 ± 2 mL10 ± 5 mL
Average survival95 ± 5 d75 ± 3 d
** P = 0.00444
(B) Summary of orthotopic mice model
PBSLPA
Tumor injection routeOrthotopicOrthotopic
LPA injection routeDaily injectionDaily injection
No. micen = 7n = 8
Average survival160 d128 d
* P = 0.012
Metastatic organsDiaphragm, peritoneal wall, mesentery, and omentumDiaphragm, peritoneal wall, mesentery, omentum, liver, and kidney
Metastatic tumor size<2 mm>5 mm
Detected in 30% miceDetected in 50% mice
Primary tumor0.169 ± 0.112 mm30.371 ± 0.23 mm3
* P = 0.0333
Ascites4 of 7 developed ascites; no mice developed ascites before 16 wk8 of 8 developed ascites; 3 of 8 developed ascites early (at 13 wk)