Table 2.

Bacterial mutation test

Strain	Treatment	μg/plate	SHetA2				SHetA3
			−S9		+S9		−S9		+S9
			Mean	SD	Mean	SD	Mean	SD	Mean	SD
TA1535	DMSO (μmol/L)	0	21	3	21	7	21	3	21	7
	0.125	50	19	9	15	10	27	3	23	3
	0.393	158	26	9	20	6	21	3	19	3
	1.245	500	18	7	22	2	19	7	19	3
	3.937	1581	21	7	19	1	18	2	16	5
	12.452	5000	16	5	22	4	20	6	17	2
	PC: NaAZ	0.5	304	54	N/A	N/A	304	54	N/A	N/A
	PC: 2AA	5	N/A	N/A	324	81	N/A	N/A	324	81
TA1537	DMSO (μmol/L)	0	15	4	21	11	15	4	21	11
	0.004	1.58	9	6	23	3	ND	ND	ND	ND
	0.012	5.0	T	T	21	2	13	1	15	3
	0.039	15.8	T	T	21	4	11	2	17	7
	0.125	50	T	T	T	T	13	5	22	3
	0.393	158	T	T	T	T	11	3	15	2
	1.245	500	ND	ND	ND	ND	11	2	16	8
	3.937	1581	ND	ND	ND	ND	6	2	6	3
	PC: 9AZ	10	673	108	N/A	N/A	673	108	N/A	N/A
	PC: BaP	5	N/A	N/A	119	5	N/A	N/A	119	5
TA98	DMSO (μmol/L)	0	30	5	45	13	30	5	45	13
	0.004	1.58	16	6	48	2	ND	ND	ND	ND
	0.012	5.0	T	T	45	8	ND	ND	ND	ND
	0.039	15.8	T	T	52	10	ND	ND	ND	ND
	0.125	50	T	T	49	8	31	6	55	8
	0.393	158	T	T	12	6	24	4	61	2
	1.245	500	ND	ND	ND	ND	27	6	55	6
	3.937	1581	ND	ND	ND	ND	22	3	62	7
	12.452	5000	ND	ND	ND	ND	18	7	57	6
	PC: 2NF	1	108	7	N/A	N/A	108	7	N/A	N/A
	PC: BaP	5	N/A	N/A	375	13	N/A	N/A	375	13
TA100	DMSO (μmol/L)	0	124	4	160	12	124	4	160	12
	0.004	1.58	136	17	136	9	ND	ND	ND	ND
	0.012	5.0	T	T	124	14	151	16	ND	ND
	0.039	15.8	T	T	171	24	138	12	ND	ND
	0.125	50	T	T	T	T	132	5	168	19
	0.393	158	ND	ND	T	T	117	14	150	12
	1.245	500	ND	ND	T	T	123	6	213	27
	3.937	1581	ND	ND	ND	ND	T	T	217	4
	PC: NaAZ	0.5	511	35	N/A	N/A	511	35	N/A	N/A
	PC: NQO	0.5	N/A	N/A	965	70	N/A	N/A	965	70
WP2 uvrA	DMSO (μmol/L)	0	19	5	33	7	19	5	33	7
	0.125	50	17	3	27	4	18	1	37	15
	0.393	158	14	3	18	8	19	3	36	4
	1.245	500	18	1	22	2	15	2	36	6
	3.937	1581	10	2	17	3	15	3	29	9
	12.452	5000	12	3	13	3	15	3	20	4
	PC: 2AA	0.5	594	45	N/A	N/A	594	45	N/A	N/A
	PC: 2AA	15	N/A	N/A	145	10	N/A	N/A	145	10

NOTE: Mean and standard deviations of the number of revertant colonies from triplicate results are presented.

Abbreviations: PC, positive control; N/A, not applicable; T, toxic as indicated by incomplete/no background lawn.