Table 1.

Characterization of CDA gene status, dCK, CDA, and RRM1 mRNA gene expression, dCK and CDA activity, and gemcitabine and bortezomib cytotoxicity in H460 and SW1573 cells

H460SW1573
RRM1 gene expression*12.9 ± 2.74.8 ± 0.1
RRM2 gene expression*13.9 ± 3.110.8 ± 1.0
5′-NT gene expression*9.3 ± 1.168.5 ± 3.2
dCK
    Gene expression*142.7 ± 26.5152.1 ± 1.2
    Activity3.1 ± 0.54.3 ± 0.7
CDA
    A79C polymorphismCCAA
    Gene expression*24.3 ± 5.22.1 ± 0.1
    Activity18.6 ± 3.51.2 ± 0.4
IC50
    Gemcitabine4.9 ± 0.14.0 ± 0.4
    Bortezomib69.0 ± 6.410.7 ± 1.1
  • *Mean values calculated in comparison with standard curves and with respect to the respective expression values of the housekeeping gene β-actin.

  • dCK and CDA activity values have been calculated as pmol/h/mg protein.

  • IC50 values have been calculated as described in Materials and Methods.