Table 1.

Antiproliferative activity of AT7519 in a panel of human tumor cell lines

TissueCell lineInhibition of proliferation IC50 (nmol/L)
Colon carcinomaHCT11682
HT29170
SW620940
Ovarian carcinomaA2780350
SK-OV-3400
Lung carcinomaA549310
NCI-H69330
NCI-H596690
NCI-H82230
Breast carcinomaMCF-740
BT-20320
MDA-MB-468340
SK-BR3140
HCC1937460
Uterine sarcomaMESSA660
LeukemiaHL60230
K562310
MOLT-4310
F36-P390
Kasumi-1250
MOLM-13170
MV4-11170
PL-21160
LymphomaGranta-519160
JEKO-1670
Ramos720
FibroblastMRC-5980
MRC-5 (nonproliferating)>10,000
  • NOTE: Exponentially growing cells were exposed to compound for 72 h before determination of viable cell number by the addition of Alamar Blue reagent. In the case of the nonproliferating MRC-5 cells, they were grown to confluence before addition of the compound to measure compound effects in the absence of proliferation. Experiments were done in triplicate and data are mean of at least two independent experiments.