Table 1.

Influence of 4-HPR and DMS on sphingolipid metabolism in MCF-7-AdrR (NCI/ADR-RES) cells treated for extended times

AgentsCell lipids (pmol 106 cells)
CeramidesSphinganineSphingosineS1P
Control
    24 h3316.3 ± 1.555 ± 6.728.8 ± 8.8
    48 h87817 ± 2.737.3 ± 1377.7 ± 20
4-HPR
    24 h93438.3 ± 3.251 ± 11.510.5 ± 1.9
    48 h6,82251.4 ± 3.018.4 ± 3.278.9 ± 32
DMS
    24 h54414.8 ± 0.66.3 ± 0.811.4 ± 1.3
    48 h1,51527.6 ± 5.83.6 ± 1.060.9 ± 30
4-HPR/DMS
    24 h1,47854.9 ± 4.25.3 ± 0.59.2 ± 1.9
    48 h14,805135.7 ± 292.2 ± 1.029 ± 7.8
  • NOTE: Cells (70% confluent) were treated with vehicle (ethanol), 4-HPR (5.0 μmol/L), DMS (5.0 μmol/L), or 4-HPR plus DMS for +24 and +48 h. Cells were then harvested for lipid analysis by LC MS/MS. The experiment was done in triplicate.