Table 3.

Selectivity for other PH domains

CompoundsAKT1 PH mKD (μmol/L)IRS1 PH mKD (μmol/L)PDK1 PH mKD (μmol/L)
PtdIns(3,4,5)P33.08 ± 0.49NDND
DPIEL5.04 ± 0.4831.56 ± 8.49NB
10.37 ± 0.040.39 ± 0.0131.28 ± 9.54
23.66 ± 0.03NB0.17 ± 0.10
31.37 ± 0.25NB3.57 ± 0.96
40.51 ± 0.060.14 ± 0.02NB
51.35 ± 0.021.74 ± 0.410.42 ± 0.17
61.62 ± 0.02NB0.98 ± 0.48
  • NOTE: Binding affinities were measured by surface plasmon resonance spectroscopy as described in Materials and Methods and are referred to as mKD (μmol/L) for measured.

  • Abbreviations: NB, no measurable binding; ND, not determined.