KD5170 is a potent inhibitor of HDAC activity cell-based assays
| Acetylation | |||
|---|---|---|---|
| Compound | Histone H3, EC50 ± SE (μmol/L) | α-Tubulin, EC50 ± SE (μmol/L) | |
| KD5170 | 0.025 μmol/L (±0.004; n = 20) | 0.325 μmol/L (±0.1; n = 6) | |
NOTE: Cellular HDAC inhibitory activity in HeLa cells was assessed in a 384-well cytoblot using an antibody that recognizes acetylated histone H3 or acetylated α-tubulin. Cells were fixed and stained after a 7-h incubation with compound under standard HeLa cell culture conditions. Values shown represent a compilation of multiple independent experiments, as indicated (Materials and Methods).