Table 1.

Zinc finger sequences selected for Taxol resistance in HeLa cells

TFSZF
Zinc finger helices*
Predicted target sites
Kd (nmol/L)
F6F5F4F3F2F1Half-site 1Half-site 2
6ZF-1-VPDPGNLVRQAGHLASQSSNLASTSGNLVRDCRDLARRSDVLVR5-GACTGATAA-GATGCCGTG-3ND
6ZF-5-VPQAGHLASQAGHLASRSDHLTTDPGALVRRSDVLVRSDHLTNH5-TGAGCGGCT-TGAGGGGTC-3ND
6ZF-8-VPRSDNLVRQAGHLASQSSNLASQSSNLVRTSGNLVRDPGHLVR5-GAGTGATGA-GAAGATGGC-3ND
6ZF-20-VPQAGHLASQSGDLRRTSGSLVRQAGHLASDCRDLARQRANLRA5-TGAGCAGTT-TGAGCCAAA-3ND
6ZF-24-VPQSGDLRRTSGNLVRQSSNLASDPGHLVRTSGHLVRQSSNLAS5-GCAGATTAA-GGCGGTTAA-31.23 ± 0.087
6ZF-30-VPQSSNLVRQRAHLERTSGELVRRSDDLVRTSGNLVRRSDKLVR5-GAAGGAGCT-GCGGATGGG-31.22 ± 0.12
3ZF-1-VPQSSHLVRTSGSLVRRSDTSSN5-GGAGTTAAG-33.34 ± 0.32
3ZF-5-VPREDNLHTTSGSLVRRSDNLVR5-TAGGTTGAG-3ND
3ZF-6-VPQSSSLVRQSSNLVRQSSHLVR5-GTAGAAGGA-3ND
3ZF-16-VPTSGSLVRRSDTLSNRSDNLVR5-GTTAAGGAG-3ND
  • NOTE: The predicted 18-bp DNA-binding site (6ZF library selections; top) and 9-bp binding site (3ZF library selections; bottom) are indicated. Predicted target DNA sequences are presented in the 5 to 8 orientation. Kd, dissociation constant, calculated by gel retardation (22); ND, not determined.

  • * Zinc finger helices are positioned in the antiparallel orientation (COOH-F6 to F1-NH2) relatively to the DNA target sequence. Amino acid position -1 to +6 of each DNA.

  • Recognition helix is shown. Top, 6ZF proteins; bottom, 3ZF proteins.