Table 1.

Ad5-hHSG mediated inhibition of tumorigenicity ex vivo and in vivo

A. Effect of Ad5-hHSG on A549 tumor growth ex vivo
Volume of tumor (mean ± SD; mm3)
7 d8 d9 d10 d11 d12 d13 d14 d15 d
Mock34.4 ± 32.828.6 ± 14.155.4 ± 41.439.7 ± 29.258.5 ± 50.592.2 ± 101.5166.2 ± 168.2253.9 ± 285.3294.6 ± 296.9
Ad5-Null18.3 ± 17.615.5 ± 16.4*25.8 ± 23.818.9 ± 14.641.4 ± 40.765.1 ± 54.215.8 ± 64.4240.2 ± 128.3295.5 ± 160.9
Ad5-hHSG0.0 ± 0.0,0.9 ± 3.1,14.0 ± 25.39.9 ± 20.425.8 ± 57.213.9 ± 43.113.2 ± 39.2,13.4 ± 41.1,15.9 ± 49.3,
B. Effect of Ad5-hHSG on HT-29 tumor growth ex vivo
Volume of tumor (mean ± SD; mm3)
4 d
6 d
8 d
10 d
12 d
14 d
16 d
18 d

Mock19.4 ± 15.434.3 ± 27.390.8 ± 83.1229.8 ± 162.8228.0 ± 155.6361.3 ± 199.7520.0 ± 300.0892.6 ± 380.4
Ad5-Null19.2 ± 15.231.1 ± 22.892.7 ± 55.9242.1 ± 134.1375.6 ± 227.3377.6 ± 212.9775.2 ± 577.1953.3 ± 632.4
Ad5-hHSG0.0 ± 0.0,1.4 ± 4.5,1.4 ± 4.5,0.0 ± 0.0,0.0 ± 0.0,0.0 ± 0.0,5.1 ± 12.0,3.3 ± 10.6,
C. Effect of Ad5-hHSG on A549 tumor growth in vivo
Volume of tumor (mean ± SD; mm3)
5 d
7 d
9 d
11 d
13 d
15 d
17 d
19 d
21 d
Mock6.5 ± 4.929.4 ± 18.863.0 ± 47.2118.3 ± 88.5243.5 ± 202.4342.5 ± 303.0454.2 ± 357.2568.9 ± 438.6579.8 ± 456.7
Ad5-Null6.9 ± 5.028.2 ± 18.2101.7 ± 47.5121.3 ± 72.9257.5 ± 219.3302.7 ± 205.9408.8 ± 230.4475.3 ± 229.0566.3 ± 230.6
Ad5-hHSG15.2 ± 14.822.0 ± 17.223.1 ± 24.9,49.8 ± 33.5,87.7 ± 51.7,114.6 ± 78.4,193.8 ± 153.7,222.4 ± 162.8256.6 ± 278.0,
D. Effect of Ad5-hHSG on HT-29 tumor growth in vivo
Volume of tumor (mean ± SD; mm3)
7 d
8 d
9 d
10 d
11 d
12 d
13 d
14 d
15 d
Mock174.1 ± 102.7323.2 ± 133.3463.1 ± 210.2657.5 ± 266.4976.0 ± 450.3853.5 ± 380.1842.0 ± 353.41025.7 ± 467.31093.5 ± 583.6
Ad5-Null185.8 ± 142.9318.3 ± 225.7403.1 ± 233.6498.8 ± 238.8692.2 ± 286.4635.7 ± 212.8661.1 ± 201.1831.2 ± 246.4822.4 ± 215.9
Ad5-hHSG149.3 ± 116.5268.9 ± 183.5330.6 ± 156.9370.0 ± 155.2525.1 ± 254.7493.3 ± 163.7495.9 ± 198.8482.6 ± 150.5,541.1 ± 191.7
  • NOTE: The volumes of tumors represent mean ± SD (n = 10). One-way ANOVA was used for statistical comparison among three groups, and post-hoc test (LSD) was used for statistical comparison between two groups.

  • * P < 0.05 Ad5-Null versus mock.

  • P < 0.05 Ad5-hHSG versus mock.

  • P < 0.05 Ad5-hHSG versus Ad5-Null.